Part:BBa_K5302013:Design

pBBR-LppOmpA-mCherry

Assembly Compatibility:

• 10
  INCOMPATIBLE WITH RFC[10]

  Illegal EcoRI site found at 3631
  Illegal EcoRI site found at 3679
  Illegal EcoRI site found at 3952
  Illegal PstI site found at 2016
  Illegal PstI site found at 3940
  
• 12
  INCOMPATIBLE WITH RFC[12]

  Illegal EcoRI site found at 3631
  Illegal EcoRI site found at 3679
  Illegal EcoRI site found at 3952
  Illegal PstI site found at 2016
  Illegal PstI site found at 3940
  Illegal NotI site found at 1057
  
• 21
  INCOMPATIBLE WITH RFC[21]

  Illegal EcoRI site found at 3631
  Illegal EcoRI site found at 3679
  Illegal EcoRI site found at 3952
  Illegal BglII site found at 1803
  Illegal BamHI site found at 3583
  Illegal XhoI site found at 4297
  
• 23
  INCOMPATIBLE WITH RFC[23]

  Illegal EcoRI site found at 3631
  Illegal EcoRI site found at 3679
  Illegal EcoRI site found at 3952
  Illegal PstI site found at 2016
  Illegal PstI site found at 3940
  
• 25
  INCOMPATIBLE WITH RFC[25]

  Illegal EcoRI site found at 3631
  Illegal EcoRI site found at 3679
  Illegal EcoRI site found at 3952
  Illegal PstI site found at 2016
  Illegal PstI site found at 3940
  Illegal NgoMIV site found at 2467
  Illegal NgoMIV site found at 2750
  Illegal NgoMIV site found at 3538
  Illegal NgoMIV site found at 4723
  Illegal AgeI site found at 4158
  Illegal AgeI site found at 4563
  
• 1000
  INCOMPATIBLE WITH RFC[1000]

  Illegal BsaI.rc site found at 1379
  Illegal SapI.rc site found at 2316
  Illegal SapI.rc site found at 2526
  

Design Notes

Please be advised that all listed genes have undergone codon optimization for our chassis strain, Escherichia coli Nissle 1917 (EcN). For those considering alternative chassis organisms, it is recommended to reassess the necessity of codon optimization.

Source

Lpp: Human OmpA: Escherichia coli (strain K12) mCherry: Mushroom coral